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Journal articleRowlands CJ, Park D, Bruns OT, et al., 2017,
Wide-field three-photon excitation in biological samples
, Light: Science and Applications, Vol: 6, Pages: 1-9, ISSN: 2047-7538Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.
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Journal articleBruns OT, Bischof TS, Harris DK, et al., 2017,
Next-generation in vivo optical imaging with short-wave infrared quantum dots
, NATURE BIOMEDICAL ENGINEERING, Vol: 1, ISSN: 2157-846X -
Journal articleAbshire JR, Rowlands CJ, Ganesan SM, et al., 2017,
Quantification of labile heme in live malaria parasites using a genetically encoded biosensor
, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 114, Pages: E2068-E2076, ISSN: 0027-8424 -
Journal articlePark JK, Rowlands CJ, So PTC, 2017,
Enhanced Axial Resolution of Wide-Field Two-Photon Excitation Microscopy by Line Scanning Using a Digital Micromirror Device
, MICROMACHINES, Vol: 8, ISSN: 2072-666X -
Journal articleUzel SGM, Platt RJ, Subramanian V, et al., 2016,
Microfluidic device for the formation of optically excitable, three-dimensional, compartmentalized motor units
, SCIENCE ADVANCES, Vol: 2, ISSN: 2375-2548- Author Web Link
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- Citations: 144
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Conference paperRowlands CJ, Bruns OT, Franke D, et al., 2016,
Near-infrared temporal focusing microscopy with quantum dot fluorophores
The tissue penetration depth of temporal focusing is increased by utilizing longer excitation wavelengths combined with quantum dots possessing extremely high two-photon crosssections. Cerebral vasculature is imaged in a mouse brain in vivo.
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Book chapterSo PTC, Yew E, Rowlands C, 2016,
Applications of Multiphoton Microscopy in Dermatology
, IMAGING IN DERMATOLOGY, Editors: Hamblin, Avci, Gupta, Publisher: ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD, Pages: 241-268, ISBN: 978-0-12-802838-4- Author Web Link
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- Citations: 2
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Journal articleRowlands CJ, Bruns OT, Bawendi MG, et al., 2015,
Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs
, JOURNAL OF BIOMEDICAL OPTICS, Vol: 20, ISSN: 1083-3668- Author Web Link
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- Citations: 7
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Conference paperXue Y, Rowlands CJ, So PTC, 2015,
Parallel and Flexible Imaging using Two-photon RESOLFT Microscopy with Spatial Light Modulator Control
, Conference on Multiphoton Microscopy in the Biomedical Sciences XV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X- Author Web Link
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- Citations: 3
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Journal articleRowlands CJ, Wu J, Uzel SGM, et al., 2014,
3D-resolved targeting of photodynamic therapy using temporal focusing
, Laser Physics Letters, Vol: 11, ISSN: 1612-2011A method for selectively inducing apoptosis in tumor nodules is presented, with close-to-cellular level resolution, using 3D-resolved widefield temporal focusing illumination. Treatment times on the order of seconds were achieved using Verteporfin as the photosensitizer, with doses of 30 μg ml−1 and below. Results were achieved on both 2D and 3D cell cultures, demonstrating that treatment was possible through approximately one hundred microns of dense tumor nodules.
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Journal articleKong K, Rowlands CJ, Varma S, et al., 2014,
Increasing the speed of tumour diagnosis during surgery with selective scanning Raman microscopy
, JOURNAL OF MOLECULAR STRUCTURE, Vol: 1073, Pages: 58-65, ISSN: 0022-2860- Author Web Link
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- Citations: 10
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Journal articleYew E, Rowlands C, So PTC, 2014,
Application of multiphoton microscopy in dermatological studies: A mini-review
, JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES, Vol: 7, ISSN: 1793-5458- Author Web Link
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- Citations: 55
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Journal articleSo PTC, Yew EYS, Rowlands C, 2013,
High-Throughput Nonlinear Optical Microscopy
, BIOPHYSICAL JOURNAL, Vol: 105, Pages: 2641-2654, ISSN: 0006-3495- Author Web Link
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- Citations: 31
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Journal articleKong K, Rowlands CJ, Varma S, et al., 2013,
Diagnosis of tumors during tissue-conserving surgery with integrated autofluorescence and Raman scattering microscopy
, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 110, Pages: 15189-15194, ISSN: 0027-8424- Author Web Link
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- Citations: 179
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Journal articleRowlands CJ, Yew EYS, So PTC, 2013,
Parallel super-resolution imaging
, NATURE METHODS, Vol: 10, Pages: 709-710, ISSN: 1548-7091- Author Web Link
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- Citations: 2
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Journal articleRowlands CJ, So PTC, 2013,
On the correction of errors in some multiple particle tracking experiments
, APPLIED PHYSICS LETTERS, Vol: 102, ISSN: 0003-6951- Author Web Link
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- Citations: 9
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Journal articleRowlands CJ, Varma S, Perkins W, et al., 2012,
Rapid acquisition of Raman spectral maps through minimal sampling: applications in tissue imaging
, JOURNAL OF BIOPHOTONICS, Vol: 5, Pages: 220-229, ISSN: 1864-063X- Author Web Link
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- Citations: 37
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Conference paperRowlands CJ, Notingher I, 2012,
Selective sampling in hyperspectral raman micro-spectroscopy: Obtaining maximum data with minimal sampling time
We present a new method for selectively sampling hyperspectral Raman maps in order to maximize the information that can be obtained for a given sample time. Example maps and comparisons with alternative methods are provided. © OSA 2012.
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Journal articleKong K, Rowlands CJ, Elsheikha H, et al., 2012,
Label-free molecular analysis of live <i>Neospora caninum</i> tachyzoites in host cells by selective scanning Raman micro-spectroscopy
, ANALYST, Vol: 137, Pages: 4119-4122, ISSN: 0003-2654- Author Web Link
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- Citations: 17
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Journal articleRowlands CJ, Elliott SR, 2011,
Improved blind-source separation for spectra
, JOURNAL OF RAMAN SPECTROSCOPY, Vol: 42, Pages: 1761-1768, ISSN: 0377-0486- Author Web Link
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- Citations: 7
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Contact details
Email: c.rowlands@imperial.ac.uk
Telephone: +44 (0)20 7594 1331
Department of Bioengineering
Royal School of Mines
Imperial College London
SW7 2AS